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Proteins were denatured in sample buffer and equal amounts were separated on SDS-PAGE and transferred to PVDF membrane (BioRad Laboratories, CA, USA)

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This polymorphism has been associated with increased risk of benign prostatic hyperplasia and prostate cancer (Rebbeck et al., 1999) and can be a useful biomarker to identify patients at higher risk for fatal prostate cancer (Liu et al., 2013)